Abundant Non-canonical dUTP Found in Primary Human Macrophages Drives Its Frequent Incorporation by HIV-1 Reverse Transcriptase
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Abundant Non-Canonical dUTP Found in Primary Human Macrophages Drives Its Frequent Incorporation by Human Immunodeficiency Virus Type 1 Reverse Transcriptase
Terminally differentiated/non-dividing macrophages contain extremely low cellular dNTP concentrations (20-40 nM), compared to activated CD4 + T cells (2-5 M). However, our LC-MS/MS study revealed that the non-canonical dUTP concentration (2.9 M) is ~60 times higher than TTP in macrophages, while the concentrations of dUTP and TTP in dividing human primary lymphocytes are very similar. Specifi...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 2011
ISSN: 0021-9258
DOI: 10.1074/jbc.m111.234047